167: SPOROCYTOPHAGA MEDIUM

Modifications: It is recommended to use the following modified medium for the reactivation of freeze-dried samples: Prepare agar plates of medium 167 by supplementing medium with 1.5% (w/v) agar. Place 4 pieces of sterile cellulose (not nitrocellulose!) filter paper on each agar plate. Suspend freeze-dried content of the inner vial of one ampoule with 0.5 ml of liquid medium 167 and inoculate each piece of the filter paper with 1 large drop of the cell suspension. Alternatively, the strain also grows with 0.1% glucose or 0.1% cellobiose as carbon source.

Growth observed:

Growth conditions: 3-7 days aerobic 25 °C